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EN 17252:2020

(Main)

Foodstuffs - Determination of phomopsin A in lupin seeds and lupin derived products by HPLC-MS/MS

Foodstuffs - Determination of phomopsin A in lupin seeds and lupin derived products by HPLC-MS/MS

This document specifies a procedure for the determination of phomopsin A in lupin seeds and lupin-derived products based on liquid chromatography with tandem mass spectrometry (LC-MS/MS). Several phomopsins exist, i.e. phomopsin A, B, C and D, but the method only deals with the quantitative measurement of phomopsin A due to lack of commercially available analytical reference standards for the other phomopsins.
The method has been validated for phomopsin A in naturally contaminated lupin seeds, lupin flour and crisp bread at levels ranging from approximately 5 µg/kg to 60 µg/kg

Lebensmittel - Bestimmung von Phomopsin A in Lupinensamen und Lupinenerzeugnissen mit HPLC-MS/MS

Dieses Dokument legt ein Verfahren zur Bestimmung von Phomopsin A in Lupinensamen und Lupinenerzeugnissen mit Flüssigchromatographie und Tandem-Massenspektrometrie (LC MS/MS, en: liquid chromatography mass spectrometry) fest. Es gibt mehrere Phomopsine, z. B. Phomopsin A, B, C und D, dieses Verfahren behandelt jedoch nur die quantitative Bestimmung von Phomopsin A, da Vergleichssubstanzen für die anderen Phomopsine nicht im Handel erhältlich sind.
Das Verfahren wurde für Phomopsin A in natürlich kontaminierten Lupinensamen, Lupinenmehl und Knäckebrot in Gehalten von etwa 5 μg/kg bis 60 μg/kg validiert.

Produits alimentaires - Détermination de la teneur en phomopsine A dans les graines de lupin et les produits dérivés du lupin par CL-SM/SM

Le présent document spécifie un mode opératoire pour le dosage de la phomopsine A dans les graines de lupin et les produits dérivés du lupin par chromatographie liquide couplée à la spectrométrie de masse en tandem (CL-SM/SM). Il existe plusieurs phomopsines, à savoir la phomopsine A, B, C et D. Toutefois, en raison de l’absence d’étalons de référence analytiques disponibles dans le commerce pour les autres phomopsines, la méthode concerne uniquement la détermination quantitative de la teneur en phomopsine A.
La méthode a été validée dans des échantillons naturellement contaminés en phomopsine A de graines de lupin, de farine de lupin et de biscottes à des teneurs allant de 5 µg/kg à 60 µg/kg environ.

Živila - Določevanje fomopsina A v semenih volčjega boba in predelanih proizvodih s HPLC-MS/MS

Ta dokument opisuje postopek za določevanje fomopsinov v semenih volčjega boba in predelanih proizvodih s tekočinsko kromatografijo s tandemsko masno spektrometrijo (LC-MS/MS). Obstaja več fomopsinov (fomopsin A, B, C in D), toda zaradi pomanjkanja komercialno razpoložljivih analitičnih referenčnih standardov za druge fomopsine metoda zajema le kvantitativno merjenje fomopsina A.
Metoda je bila validirana za fomopsin A v naravno kontaminiranih semenih volčjega boba, moki volčjega boba in hrustljavem kruhu v razponu od približno 5 do 60 µg/kg.

General Information

Status
Published
Publication Date
07-Jan-2020
Withdrawal Date
30-Jul-2020
ICS
67.060 - Cereals, pulses and derived products
Technical Committee
CEN/TC 275 - Food analysis - Horizontal methods
Drafting Committee
CEN/TC 275/WG 5 - Biotoxins
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
08-Jan-2020
Due Date
15-Aug-2019
Completion Date
08-Jan-2020
Directive
882/2004 - Regulation (EC) No 882/2004 of the European Parliament and of the Council of 29 April 2004 onofficial controls performed to ensure the verification of compliance with feed and food law, animal health and animal welfare rules
Mandate
M/520 - Mandate for standardisation addressed to CEN for methods of analysis for mycotoxins in food
Ref Project
SIST EN 17252:2020 - Foodstuffs - Determination of phomopsin A in lupin seeds and lupin derived products by HPLC-MS/MS

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SLOVENSKI STANDARD
SIST EN 17252:2020
01-marec-2020
Živila - Določevanje fomopsina A v semenih volčjega boba in predelanih
proizvodih s HPLC-MS/MS
Foodstuffs - Determination of phomopsin A in lupin seeds and lupin derived products by
HPLC-MS/MS
Lebensmittel - Bestimmung von Phomopsin A in Lupinensamen und
Lupinenerzeugnissen mit LC-MS/MS
Produits alimentaires - Détermination de la teneur en phomopsine A dans les graines de
lupin et les produits dérivés du lupin par CL-SM/SM
Ta slovenski standard je istoveten z: EN 17252:2020
ICS:
67.060 Žita, stročnice in proizvodi iz Cereals, pulses and derived
njih products
SIST EN 17252:2020 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN 17252:2020

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SIST EN 17252:2020


EN 17252
EUROPEAN STANDARD

NORME EUROPÉENNE

January 2020
EUROPÄISCHE NORM
ICS 67.060
English Version

Foodstuffs - Determination of phomopsin A in lupin seeds
and lupin derived products by HPLC-MS/MS
Produits alimentaires - Détermination de la teneur en Lebensmittel - Bestimmung von Phomopsin A in
phomopsine A dans les graines de lupin et les produits Lupinensamen und Lupinenerzeugnissen mit
dérivés du lupin par CL-SM/SM LC-MS/MS
This European Standard was approved by CEN on 9 October 2019.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATIO N

EUROPÄISCHES KOMITEE FÜR NORMUN G

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 17252:2020 E
worldwide for CEN national Members.

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SIST EN 17252:2020
EN 17252:2020 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 5
5 Reagents . 5
6 Apparatus and equipment . 7
7 Procedure. 8
8 Calculation . 10
9 Precision . 10
10 Test report . 11
Annex A
...

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