SIST EN 14104:2003

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.ORþHYDQMHErzeugnisse aus pflanzlichen und tierischen Fetten und Ölen - Fettsäure-Methylester (FAME) - Bestimmung der SäurezahlProduits dérivés des corps gras - Esters méthyliques d'acides gras (EMAG) - Détermination de l'indice d'acideFat and oil derivatives - Fatty Acid Methyl Esters (FAME) - Determination of acid value67.200.10Animal and vegetable fats and oilsICS:Ta slovenski standard je istoveten z:EN 14104:2003SIST EN 14104:2003en01-november-2003SIST EN 14104:2003SLOVENSKI
STANDARD



SIST EN 14104:2003



EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 14104April 2003ICS 67.200.10English versionFat and oil derivatives - Fatty Acid Methyl Esters (FAME) -Determination of acid valueProduits dérivés des corps gras - Esters méthyliquesd'acides gras (EMAG) - Détermination de l'indice d'acideErzeugnisse aus pflanzlichen und tierischen Fetten undÖlen - Fettsäure-Methylester (FAME) - Bestimmung derSäurezahlThis European Standard was approved by CEN on 2 January 2003.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,Hungary, Iceland, Ireland, Italy, Luxembourg, Malta, Netherlands, Norway, Portugal, Slovakia, Spain, Sweden, Switzerland and UnitedKingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36
B-1050 Brussels© 2003 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 14104:2003 ESIST EN 14104:2003



EN 14104:2003 (E)2ForewordThis document (EN 14104:2003) has been prepared by Technical Committee CEN/TC 307 "Oilseeds, vegetableand animal fats and oils and their by-products - Methods of sampling and analysis", the secretariat of which is heldby AFNOR.This European Standard shall be given the status of a national standard, either by publication of an identical text orby endorsement, at the latest by October 2003 and conflicting national standards shall be withdrawn at the latest byOctober 2003.This document has been prepared under Mandate M/245 on Fatty Acid Methylester (FAME) given to CEN by theEuropean Commission and the European Free Trade Association.Annex A is informative.According to the CEN/CENELEC Internal Regulations, the national standards organizations of the followingcountries are bound to implement this European Standard: Austria, Belgium, Czech Republic, Denmark, Finland,France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Luxembourg, Malta, Netherlands, Norway, Portugal,Slovakia, Spain, Sweden, Switzerland and the United Kingdom.SIST EN 14104:2003



EN 14104:2003 (E)3IntroductionThis European Standard is based on the EN ISO 660 [1] which was specifically adapted for the determination ofacid value of fatty acid methyl esters (FAME).SIST EN 14104:2003



EN 14104:2003 (E)41 ScopeThis European Standard specifies one titrimetric method for the determination of acid value in light coloured FattyAcid Methyl Esters, hereinafter referred as FAME.It allows the determination of acid value within a range of 0,10 mg KOH/g to 1,00 mg KOH/g.2 Normative referencesThis European Standard incorporates by dated or undated reference, provisions from other publications. Thesenormative references are cited at the appropriate places in the text, and the publications are listed hereafter. Fordated references, subsequent amendments to or revisions of any of these publications apply to this EuropeanStandard only when incorporated in it by amendment or revision. For undated references the latest edition of thepublication referred to applies (including amendments).EN ISO 661, Animal and vegetable fats and oils - Preparation of test sample (ISO 661:1989).EN ISO 3696, Water for analytical laboratory use - Specification and test methods (ISO 3696:1987).3 Terms and definitionsFor the purposes of this European Standard, the following term and definition apply.3.1acid valuenumber of milligrams of potassium hydroxide required to neutralise the free fatty acids present in 1 g of FAME,when determined in accordance with the procedure specified in this European StandardAcid value is expressed in milligrams of potassium hydroxide per gram of sample.NOTEIf the sample contains mineral acids these are, by convention determined as a part of total acid value. This methoddoes not allow to distinguish between weak (from free fatty acids) and strong (from mineral acids, if present) acidity.4 PrincipleA test portion is dissolved in a mixed solvent and titrated with a diluted solution of potassium hydroxide, usingphenolphthalein as an indicator in order to detect the titration end point.5 ReagentsUse only reagents of recognised analytical grade and water of grade 3 in accordance with EN ISO 3696.5.1Diethyl ether and 95 % ethanol, 1 + 1 mixture by volume.WARNINGDiethyl ether is very flammable and may form explosive peroxides. Use with great caution.Neutralise, just before use, by adding the potassium hydroxide solution (5.2) in the presence of 0,3 ml ofphenolphthalein alcoholic solution (5.3) per 100 ml of solvent mixture.If it is not possible to use diethyl ether, a mixed solvent may be used as follows:¾ toluene and 95 % (V/V) ethanol, 1 + 1 mixture by volume;¾ toluene and 99 % (V/V) 2-propanol, 1 + 1 mixture by volume.SIST EN 14104:2003



EN 14104:2003 (E)5The mixed solvent can be replaced by 99 % (V/V) 2 propanol.NOTELarger volumes of solvent mixture and indicator may be necessary for dark-coloured samples.5.2Potassium hydroxide, standard volumetric solution in ethanol:mol/l 10(KOH),=cThe ethanolic potassium hydroxide solution can be replaced by aqueous potassium or sodium hydroxide solutions,but only if the volume of water introduced does not lead to phase separation.5.2.1Preparation of the solutionDissolve approximately 7 g of potassium hydroxide pellets in ethanol and dilute to 1 000 ml with the same solvent.NOTE2 propanol c
...