ISO/DIS 13496

DRAFT INTERNATIONAL STANDARD
ISO/DIS 13496
ISO/TC 34/SC 6 Secretariat: SAC
Voting begins on: Voting terminates on:
2021-01-14 2021-04-08
Meat and meat products — Detection and determination of
colouring agents
ICS: 67.120.10
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ISO/DIS 13496:2021(E)
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ISO/DIS 13496:2021(E)
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Foreword ........................................................................................................................................................................................................................................iv

1 Scope ................................................................................................................................................................................................................................. 1

2 Normative references ...................................................................................................................................................................................... 1

3 Terms and definitions ..................................................................................................................................................................................... 2

4 Principle ........................................................................................................................................................................................................................ 2

4.1 Thin-layer chromatography ......................................................................................................................................................... 2

4.2 HPLC ................................................................................................................................................................................................................. 2

5 Sampling ........................................................................................................................................................................................................................ 2

6 Preparation of test sample ......................................................................................................................................................................... 2

7 Test method of thin-layer chromatography .............................................................................................................................. 2

7.1 Reagents........................................................................................................................................................................................................ 2

7.2 Apparatus .................................................................................................................................................................................................... 4

7.3 Procedure .................................................................................................................................................................................................... 4

7.3.1 Test portion .......................................................................................................................................................................... 4

7.3.2 Fatty samples ...................................................................................................................................................................... 5

7.3.3 Non-fatty samples ........................................................................................................................................................... 5

7.3.4 Transfer of the colours to polyamide powder ........................................................................................ 5

7.3.5 Elution and concentration of isolated colours ....................................................................................... 5

7.3.6 Thin-layer chromatographic separation ..................................................................................................... 6

7.3.7 Confirmation ....................................................................................................................................................................... 6

8 Test method of HPLC ......................................................................................................................................................................................... 6

8.1 Reagents........................................................................................................................................................................................................ 6

8.2 Apparatus .................................................................................................................................................................................................... 7

8.3 Procedure .................................................................................................................................................................................................... 7

8.3.1 Test portion .......................................................................................................................................................................... 7

8.3.2 Fatty samples ...................................................................................................................................................................... 7

8.3.3 Non-fatty samples ........................................................................................................................................................... 7

8.3.4 Transfer of the colours to polyamide powder ........................................................................................ 7

8.3.5 Elution and concentration of isolated colours ....................................................................................... 8

8.3.6 HPLC analysis...................................................................................................................................................................... 8

8.4 Calculation .................................................................................................................................................................................................. 9

8.5 Precision ....................................................................................................................................................................................................... 9

8.6 Limit of detection (LOD) and limit of quantification (LOQ) ............................................................................. 9

9 Test report ................................................................................................................................................................................................................... 9

colouring agents .................................................................................................................................................................................................10

Annex B (normative) Possible interference by colours .................................................................................................................11

Annex C (informative) Absorbance spectra ................................................................................................................................................12

Annex D (informative) Chromatogram and wavelength ................................................................................................................14

Annex E (informative) Interlaboratory testing .......................................................................................................................................15

Bibliography .............................................................................................................................................................................................................................53

ISO (the International Organization for Standardization) is a worldwide federation of national standards

bodies (ISO member bodies). The work of preparing International Standards is normally carried out

through ISO technical committees. Each member body interested in a subject for which a technical

committee has been established has the right to be represented on that committee. International

organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.

ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of

The procedures used to develop this document and those intended for its further maintenance are

described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the

different types of ISO documents should be noted. This document was drafted in accordance with the

editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).

Attention is drawn to the possibility that some of the elements of this document may be the subject of

patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of

any patent rights identified during the development of the document will be in the Introduction and/or

Attention is drawn to the possibility that some of the elements of this International Standard may be the

subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights.

International Standard ISO 13496 was prepared by Technical Committee ISO/TC 34, Food products,

Annex B forms a normative part of this International Standard. Annexes A and C are for information only.

This edition cancels and replaces the edition (ISO13496-2000), which has been technically revised.

This document specifies detection method using thin-layer chromatography and determination method

using high performance liquid chromatography (HPLC) for colouring agents in meat and meat products.

This document is applicable to meat and meat products, including livestock and poultry products.

The method using thin-layer chromatography can detect the following colouring agents:

Synonyms and identity numbers of these colouring agents are listed in Annex A. The plant colours

and plant extracts which have been observed not to interfere with this method are listed in Table B.1.

Natural colours which in some cases have been shown to interfere with this method are listed in

The following normative documents contain provisions which, through reference in this text,

constitute provisions of this International Standard. For dated references, subsequent amendments

to, or revisions of, any of these publications do not apply. However, parties to agreements based on

this International Standard are encouraged to investigate the possibility of applying the most recent

editions of the normative documents indicated below. For undated references, the latest edition of the

normative document referred to applies. Members of ISO and IEC maintain registers of currently valid

ISO 3696:1987, Water for analytical laboratory use — Specification and test methods

detection of the presence or absence of colouring agents in accordance with the method specified in

The colouring agents are extracted from a test portion with hot water and adsorbed onto polyamide

powder. The extracted colouring agents are purified by column chromatography and the colours are

eluted from the column. The colouring agents are identified by thin-layer chromatography.

The colouring agents are extracted from a test portion with hot water and adsorbed onto polyamide

powder. The extracted colouring agents are injected into the column and chromatographed in HPLC in

reverse phase (RP). The colouring agents are identified according to retention time and quantified with

It is important that the laboratory receive a sample which is truly representative and has not been

Proceed from a representative sample of at least 200 g. Store the sample in such a way that deterioration

Homogenize the laboratory sample with the appropriate equipment (7.2.1). Take care that the

temperature of the sample material does not rise above 25 °C. If a mincer is used, pass the sample at

Fill a suitable airtight container with the prepared sample. Close the container and store in such a way

that deterioration and change in composition of the sample are prevented. Analyse the sample as soon

7.1.14 Sand, fine granular, hydrochloric acid-washed, neutralized and calcinated.

The purities of the standard colours may vary so it is necessary to know the purity of the colours to be

Separately make solutions in water of each of the standard reference colours (7.1.15) with a standard

Prepare solutions of indigotine on the day of use. Other solutions will keep for at least 3 months

Weigh, to the nearest 0,1 g, 25 g of trisodium citrate dihydrate (7.1.6) into a 1 000 ml one-mark

Mix 80 volumes of this citrate solution with 20 volumes of ammonia solution (7.1.4) and 12 volumes of

To avoid or reduce interference from safflor or saffran, it is advisable to use chromatography solution II

Mix 6 volumes of propan-1-ol (7.1.7) with 1 volume of ethyl acetate (7.1.8) and 3 volumes of water.

Mix 50 volumes of 2-methyl-2-propanol (7.1.9) with 12 volumes of propionic acid (7.1.10) and 38 volumes

7.2.1 Mechanical or electrical homogenizing equipment, capable of homogenizing the

Use a high-speed rotational cutter, or a mincer fitted with a plate with apertures not exceeding 4,0 mm

7.2.7 Chromatographic column, of glass, with fritted filter and tap, of length about 20 cm, diameter

about 30 mm, filter pore size 40 µm to 100 µm (porosity grade P 100 according to ISO 4793 ).

7.2.9 Thin-layer plates, coated with a layer of cellulose powder of 0,10 mm thickness, or equivalent.

WARNING — If the sample contains indigotine, the temperature shall not at any time during

the analysis exceed 35 °C. Indigotine partially decomposes in chromatography solution I, so

WARNING — Erythrosine is sensitive to light. When pausing in the course of the analysis,

solutions and plates shall be stored in the dark. The same also holds for indigotine.

Weigh, to the nearest 0,1 g, 5 g of the prepared test sample (see clause 6) into a centrifuge tube (7.2.2).

Add about 20 ml of petroleum ether (7.1.2) to the centrifuge tube and mix with a glass rod. Decant the

Add 25 ml of boiling water (see warning above) and mix. Add 25 ml of the eluent solution (7.1.11).

Check that the pH is 9±0,5 using the pH-meter (7.2.11). If not, adjust the pH with acetic acid (7.1.5) or

Decant the clear solution into a flat-bottomed flask (7.2.3). In the case of indigotine, use a round-

Add 5 ml of water to the centrifuge tube containing the residue. Mix and add 10 ml of the eluent solution

Repeat the procedure until all colour has been extracted from the sample then combine all extracts.

Evaporate the combined extract on a water bath to about 25 ml in order to remove methanol. In the

case of indigotine, use a round-bottomed flask (7.2.4) and the rotary evaporator (7.2.6) at 35 °C.

Using acetic acid (7.1.5) or ammonia solution (7.1.4).adjust the pH to between 4 and 5.

Add 1 g of polyamide powder (7.1.13) to the warm solution (see warnings). Shake vigorously for 1 min.

Check that no colour remains in the solution. If the solution is coloured, add some more polyamide

NOTE Some natural colours (see Annex B) are not entirely adsorbed on the polyamide powder, leaving

the solution coloured even if all synthetic colours have been completely adsorbed. It is usually possible

to decide from the type of sample whether or not such natural colours are present.

Rinse the flat-bottomed flask with three 10 ml portions of hot water (see warnings) and add the

rinsings, portion by portion, to the column. Wash the column another three times with 10 ml portions

of hot water (see warnings) and finally three times with 5 ml of methanol (7.1.3). If natural colours are

eluted, continue washing the column with methanol until the eluted methanol is colourless.

Place a flask (7.2.4) under the column and elute the colours from the polyamide powder with 5 ml

portions of the eluent solution (7.1.11), at an elution volume flow rate of 2 ml/min, until the polyamide

Evaporate the eluate to dryness using the evaporator (7.2.6) at a temperature of at most 35 °C (see

Add 1,0 ml or 2,0 ml of eluent solution (7.1.11) depending on the amount and number of colours and

dissolve the residue. Transfer the colour solution to a plastics container (7.2.8).

Prepare three standard reference thin-layer chromatographic plates. Using a micropipette (7.2.10),

dispense a spot of about 5 µl (diameter < 5 mm) of each standard solution (7.1.16) separately on each

plate (7.2.9). Develop these separately, one with each chromatography eluent (7.1.17, 7.1.18 and 7.1.19)

in an unsaturated tank until the solvent front is about 10 cm to 12 cm from the starting line. Remove

the plates from the tank and dry in air under a hood. Store the plates in the dark. The spots, except for

Using a micropipette (7.2.10), apply to a thin-layer plate (7.2.9) a just-visible amount of sample solution

Develop the plate in an unsaturated tank to a height of approximately 10 cm to 12 cm using a suitable

chromatography solution (7.1.16, 7.1.17 or 7.1.18); i.e. the solution which gives the best separation of

the colours detected in the sample (see clause 1). Sometimes it will be necessary to prepare a second

sample plate and develop this in one of the other two eluents to obtain the best separation.

Compare the sample spots with the appropriate standard reference plate (7.3.6.1).

It is recommended that different amounts of sample solutions be applied in the case of mixtures of

colorants, because colorants may be present in various concentrations in the concentrate.

Tailing is usually caused by inadequate purification. If this is the case, adsorb the colorant again with

the adsorbent, wash with hot water and remove the adsorbent as previously described.

Confirm the identity of the colorants by chromatographing the concentrate (7.3.6.2) in a mixture of

In case of doubt, elute the colorant from the plate with a neutral solution (water or ethanol, or 0,2 g/l

ammonium acetate solution), an acid (0,1 mol/l hydrochloric acid) and an alkali (0,1 mol/l sodium

hydroxide solution) and compare the absorption spectrum of the colorant to that of the standard. See

Weigh 1,54 g of ammonium acetate (8.1.2), add appropriate water to dissolve, and dilute to 1000 ml

Separately make solutions in 10 % methanol (8.1.4) of each of the standard reference colours (7.1.15)

Diluted the 1 mg/ml stock solutions (8.1.5) by 20 times with 10 % methanol (8.1.4) and filter through

8.2.1 HPLC chromatographic system, with column thermostat and UV/visible or diode array

WARNING — If the sample contains indigotine, the temperature shall not at any time during the

WARNING — Erythrosine is sensitive to light. When pausing in the course of the analysis,

Weigh, to the nearest 0,001 g, 5 g of the prepared test sample (see clause 6) into a centrifuge tube (7.2.2).

Place a flask (7.2.4) under the column and elute the colours from the polyamide powder with 5 ml

portions of the eluent solution (7.1.11), at an elution volume flow rate of 2 ml/min, until the polyamide

Evaporate the eluate to dryness using the evaporator (7.2.6) at a temperature of at most 35 °C (see

Add 1,0 ml or 2,0 ml of water (7.1.1) depending on the amount and number of colours and dissolve the

residue. Filter colour solution through 0,45 µm microporous membrane (8.2.2)for injection into HPLC

f) Wavelength range of diode array detector: 400 nm-800 nm, or wavelength of UV detector detection:

Under above determination condition, when the retention time for the peak of analyte in unknown

sample is same as the retention time of the standard, we can judge there has synthetical pigments in

the sample. Chromatogram of synthetical pigments standard is in Annex D. The method quantified by

the external standard curve. The responses of synthetical pigments in the sample solution should be in