ISO/TS 12869-2:2024

FINAL
TECHNICAL ISO/DTS
DRAFT
SPECIFICATION 12869-2
ISO/TC 147/SC 4
Water quality — Detection and
Secretariat: DIN
quantification of Legionella spp.
Voting begins on:
2023-10-30 and/or Legionella pneumophila by
concentration and genic amplification
Voting terminates on:
2023-12-25
by quantitative polymerase chain
reaction (qPCR) —
Part 2:
On-site methods
Qualité de l'eau — Détection et quantification de Legionella spp.
et/ou Legionella pneumophila par concentration et amplification
génique par réaction de polymérisation en chaîne quantitative
(qPCR) —
Partie 2: Méthodes sur site
RECIPIENTS OF THIS DRAFT ARE INVITED TO
SUBMIT, WITH THEIR COMMENTS, NOTIFICATION
OF ANY RELEVANT PATENT RIGHTS OF WHICH
THEY ARE AWARE AND TO PROVIDE SUPPOR TING
DOCUMENTATION.
IN ADDITION TO THEIR EVALUATION AS
Reference number
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO-
ISO/DTS 12869-2:2023(E)
LOGICAL, COMMERCIAL AND USER PURPOSES,
DRAFT INTERNATIONAL STANDARDS MAY ON
OCCASION HAVE TO BE CONSIDERED IN THE
LIGHT OF THEIR POTENTIAL TO BECOME STAN-
DARDS TO WHICH REFERENCE MAY BE MADE IN
NATIONAL REGULATIONS. © ISO 2023

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ISO/DTS 12869-2:2023(E)
FINAL
TECHNICAL ISO/DTS
DRAFT
SPECIFICATION 12869-2
ISO/TC 147/SC 4
Water quality — Detection and
Secretariat: DIN
quantification of Legionella spp.
Voting begins on:
and/or Legionella pneumophila by
concentration and genic amplification
Voting terminates on:
by quantitative polymerase chain
reaction (qPCR) —
Part 2:
On-site methods
Qualité de l'eau — Détection et quantification de Legionella spp.
et/ou Legionella pneumophila par concentration et amplification
génique par réaction de polymérisation en chaîne quantitative
(qPCR) —
Partie 2: Méthodes sur site
COPYRIGHT PROTECTED DOCUMENT
© ISO 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
RECIPIENTS OF THIS DRAFT ARE INVITED TO
ISO copyright office
SUBMIT, WITH THEIR COMMENTS, NOTIFICATION
OF ANY RELEVANT PATENT RIGHTS OF WHICH
CP 401 • Ch. de Blandonnet 8
THEY ARE AWARE AND TO PROVIDE SUPPOR TING
CH-1214 Vernier, Geneva
DOCUMENTATION.
Phone: +41 22 749 01 11
IN ADDITION TO THEIR EVALUATION AS
Reference number
Email: copyright@iso.org
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO-
ISO/DTS 12869-2:2023(E)
Website: www.iso.org
LOGICAL, COMMERCIAL AND USER PURPOSES,
DRAFT INTERNATIONAL STANDARDS MAY ON
Published in Switzerland
OCCASION HAVE TO BE CONSIDERED IN THE
LIGHT OF THEIR POTENTIAL TO BECOME STAN-
DARDS TO WHICH REFERENCE MAY BE MADE IN
ii
  © ISO 2023 – All rights reserved
NATIONAL REGULATIONS. © ISO 2023

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ISO/DTS 12869-2:2023(E)
Contents Page
Foreword .v
Introduction . vi
1 Scope . 1
2 Normative references . 1
3 Terms, definitions, symbols and abbreviated terms . 2
3.1 Terms and definitions . 2
3.2 Symbols and abbreviated terms . 3
4 Principle . 4
5 Sampling .
...

© ISO 2023 – All rights reserved
ISO/TC 147/SC 4
Date: 2023-05
ISO/DTS TS 12869--2:2023(E)
ISO/TC 147/SC 4
Secretariat: DIN
Water quality — Detection and quantification of Legionella spp. and/or Legionella pneumophila
by concentration and genic amplification by quantitative polymerase chain reaction (qPCR) —
Part 2: On-site methods
Qualité de l'eau — Détection et quantification de Legionella spp. et/ou Legionella pneumophila par
concentration et amplification génique par réaction de polymérisation en chaîne quantitative (qPCR) –
Partie 2: Méthodes sur site

DTS stage

Warning for WDs and CDs
This document is not an ISO International Standard. It is distributed for review and comment. It is subject to
change without notice and may not be referred to as an International Standard.
Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of
which they are aware and to provide supporting documentation.

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© ISOFirst edition
Date: 2023-10-16

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ISO/TS 12869-2:2023(E)
© ISO 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no
part of this publication may be reproduced or utilized otherwise in any form or by any means,
electronic or mechanical, including photocopying, or posting on the internet or an intranet, without
prior written permission. Permission can be requested from either ISO at the address below or
ISO’sISO's member body in the country of the requester.
ISO Copyright Office
CP 401 • CH. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: + 41 22 749 01 11
Email: copyright@iso.org copyright@iso.org
Website: www.iso.orgwww.iso.org
Published in Switzerland.
ii © ISO 2023 – All rights reserved

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ISO/TS 12869-2:2023(E)
Contents
Foreword . 5
Introduction . 6
1 Scope . 1
2 Normative references . 1
3 Terms, definitions, symbols and abbreviated terms . 2
3.1 Terms and definitions . 2
3.2 Symbols and abbreviated terms . 3
4 Principle . 4
5 Sampling . 4
6 General testing conditions . 5
6.1 General . 5
6.2 End users . 5
6.2.1 General . 5
6.2.2 Manufacturer’s instructions . 6
6.2.3 Proficiency . 7
6.2.4 Usability validation (human factors testing [HFT]) . 7
6.3 Premises . 7
6.3.1 Manufacturer premises . 7
6.3.2 End user premises . 8
6.4 Apparatus and consumables (excluding reagents) . 8
6.4.1 General
...