Foodstuffs - HPLC method for the determination of xanthophylls in fish flesh - Part 1: Determination of astaxanthin and canthaxanthin

This Technical Specification specifies a method for the determination of canthaxanthin and astaxanthin in fish flesh by high performance liquid chromatography (HPLC). The method can be applied at a range above 0,02 mg/kg. The method should not be applied to the determination of canthaxanthin in poultry tissues, egg yolks and shrimp tissues due to a possible interference of canthaxanthin with cryptoxanthin and xanthophyll esters sometimes present in these matrices.

Lebensmittel - HPLC-Verfahren für die Bestimmung von Xanthophyllen in Fischfleisch - Teil 1: Bestimmung von Astaxanthin und Canthaxanthin

Diese Technische Spezifikation legt ein Verfahren zur Bestimmung von Canthaxanthin und Astaxanthin in Fischfleisch mit Hochleistungsflüssigchromatographie (HPLC) fest. Das Verfahren kann bei Gehalten von mehr als 0,02 mg/kg angewendet werden. Das Verfahren sollte nicht zur Bestimmung von Canthaxanthin in Geflügelfleisch, Eigelb und Garnelenfleisch angewendet werden, weil Canthaxanthin mit Cryptoxanthin und Xanthophyllestern, die in diesen Matrices zuweilen vorliegen, interferieren kann.

Produits alimentaires - Méthode de dosage des xanthophylles dans la chair de poisson par CLHP - Partie 1: Dosage de l'astaxanthine et de la canthaxantine

La présente Spécification technique spécifie une méthode de dosage de la canthaxanthine et de
l’astaxanthine dans la chair de poisson par chromatographie liquide à haute performance (CLHP). La
méthode peut être appliquée à une gamme supérieure à 0,02 mg/kg. Il convient de ne pas appliquer la
méthode au dosage de la canthaxanthine dans les tissus de volailles, les jaunes d'oeufs et les tissus de
crevettes en raison d’une éventuelle interférence de la canthaxanthine avec les esters de cryptoxanthine et de
xanthophylle parfois présents dans ces matrices.

Živila - Metoda HPCL za določevanje ksantofilov v ribjem mesu - 1. del: Določevanje astaksantina in kantaksantina

Ta tehnična specifikacija opredeljuje metodo določevanja kantaksantina in astaksantina v ribjem mesu s tekočinsko kromatografijo visoke ločljivosti (HPLC). Metoda se lahko uporablja v razponu nad 0,02 mg/kg. Metoda se ne sme uporabljati za določevanje kantaksantina v perutninskih tkivih, jajčnih rumenjakih in tkivih kozic (morskih rakcev) zaradi morebitne interference kantaksantina s kriptoksantinovimi in ksantofilnimi estri, ki so včasih v teh matrikah.

General Information

Status
Published
Publication Date
12-Jul-2011
Current Stage
9093 - Decision to confirm - Review Enquiry
Completion Date
16-Sep-2014

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Standards Content (Sample)

SLOVENSKI STANDARD
SIST-TS CEN/TS 16233-1:2011
01-oktober-2011
äLYLOD0HWRGD+3&/]DGRORþHYDQMHNVDQWRILORYYULEMHPPHVXGHO
'RORþHYDQMHDVWDNVDQWLQDLQNDQWDNVDQWLQD
Foodstuffs - HPLC method for the determination of xanthophylls in fish flesh - Part 1:
Determination of astaxanthin and canthaxanthin
Lebensmittel - HPLC-Verfahren für die Bestimmung von Xanthophyllen in Fischfleisch -
Teil 1: Bestimmung von Astaxanthin und Canthaxanthin
Produits alimentaires - Méthode de dosage des xanthophylles dans la chair de poisson
par CLHP - Partie 1: Dosage de l'astaxanthine et de la canthaxantine
Ta slovenski standard je istoveten z: CEN/TS 16233-1:2011
ICS:
67.050 Splošne preskusne in General methods of tests and
analizne metode za živilske analysis for food products
proizvode
67.120.30 Ribe in ribji proizvodi Fish and fishery products
SIST-TS CEN/TS 16233-1:2011 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST-TS CEN/TS 16233-1:2011

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SIST-TS CEN/TS 16233-1:2011


TECHNICAL SPECIFICATION
CEN/TS 16233-1

SPÉCIFICATION TECHNIQUE

TECHNISCHE SPEZIFIKATION
July 2011
ICS 67.120.30
English Version
Foodstuffs - HPLC method for the determination of xanthophylls
in fish flesh - Part 1: Determination of astaxanthin and
canthaxanthin
Produits alimentaires - Méthode de dosage des Lebensmittel - HPLC-Verfahren für die Bestimmung von
xanthophylles dans la chair de poisson par CLHP - Partie 1: Xanthophyllen in Fischfleisch - Teil 1: Bestimmung von
Dosage de l'astaxanthine et de la canthaxantine Astaxanthin und Canthaxanthin
This Technical Specification (CEN/TS) was approved by CEN on 28 May 2011 for provisional application.

The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to submit their
comments, particularly on the question whether the CEN/TS can be converted into a European Standard.

CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS available
promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in parallel to the CEN/TS)
until the final decision about the possible conversion of the CEN/TS into an EN is reached.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.





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© 2011 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 16233-1:2011: E
worldwide for CEN national Members.

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SIST-TS CEN/TS 16233-1:2011
CEN/TS 16233-1:2011 (E)
Contents Page
Foreword .3
1 Scope .4
2 Normative references .4
3 Principle .4
4 Reagents .4
5 Apparatus .6
6 Calibration .7
7 Procedure .8
8 HPLC .9
9 Calculation . 10
10 Test report .
...

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