Animal feeding stuffs: Methods of sampling and analysis - Determination of Deoxynivalenol, Aflatoxin B1, Fumonisin B1 & B2, T-2 & HT-2 toxins, Zearalenone and Ochratoxin A in feed materials and compound feed by LC-MS/MS

This document's method of analysis is applicable for the determination of:
-   deoxynivalenol (DON) in the tested range of 100 µg/kg to 3 300 µg/kg,
-   aflatoxin B1 (AfB1) in the tested range of 2,5 µg/kg to 440 µg/kg,
-   fumonisin B1 (FB1) in the tested range of 690 µg/kg to 7 500 µg/kg,
-   fumonisin B2 (FB2) in the tested range of 200 µg/kg to 2 500 µg/kg,
-   T-2 toxin in the tested range of 7,5 µg/kg to 360 µg/kg,
-   HT-2 toxin in the tested range of 14 µg/kg to 1 800 µg/kg,
-   zearalenone (ZEN) in the tested range of 30 µg/kg to 600 µg/kg, and
-   ochratoxin A (OTA) in the tested range of 10 µg/kg to 230 µg/kg
in cereals and cereal-based compound feed by liquid-chromatography tandem mass spectrometry (LC-MS/MS). The actual working ranges could extend beyond the tested ranges.

Futtermittel: Probenahme- und Untersuchungsverfahren - Bestimmung von Deoxynivalenol, Aflatoxin B1, Fumonisin B1 und B2, T-2- und HT-2-Toxine, Zearalenon und Ochratoxin A in Einzelfuttermitteln und Mischfuttermitteln mittels LC-MS/MS

Das Analyseverfahren dieses Dokumentes ist gültig für die Bestimmung von
—   Deoxynivalenol (DON) im untersuchten Bereich von 100 µg/kg bis 3 300 µg/kg,
—   Aflatoxin B1 (AfB1) im untersuchten Bereich von 2,5 µg/kg bis 440 µg/kg,
—   Fumonisin B1 (FB1) im untersuchten Bereich von 690 µg/kg bis 7 500 µg/kg,
—   Fumonisin B2 (FB2) im untersuchten Bereich von 200 µg/kg bis 2 500 µg/kg,
—   T 2 Toxin im untersuchten Bereich von 7,5 µg/kg bis 360 µg/kg,
—   HT 2 Toxin im untersuchten Bereich von 14 µg/kg bis 1 800 µg/kg,
—   Zearalenon (ZEN) im unter¬suchten Bereich von 30 µg/kg bis 600 µg/kg und
—   Ochratoxin A (OTA) im untersuchten Bereich von 10 µg/kg bis 230 µg/kg
in Getreide und Getreidemisch¬futtermittel durch Flüssigchromatographie mit Tandem Massen-spektrometrie (LC MS/MS). Die tatsächlichen Arbeitsbereiche könnten über die geprüften Bereiche hinausgehen.

Aliments des animaux : Méthodes d’échantillonnage et d’analyse — Détermination du déoxynivalénol, de l’aflatoxine B1, de la fumonisine B1 et B2, des toxines T-2 et HT-2, de la zéaralénone et de l’ochratoxine A dans les matières premières pour aliments et les aliments composés pour animaux par CL-SM/SM

La méthode d’analyse décrite dans le présent document s’applique à la détermination :
—   du déoxynivalénol (DON) dans la plage soumise à essai allant de 100 µg/kg à 3 300 µg/kg ;
—   de l’aflatoxine B1 (AfB1) dans la plage soumise à essai allant de 2,5 µg/kg à 440 µg/kg ;
—   de la fumonisine B1 (FB1) dans la plage soumise à essai allant de 690 µg/kg à 7 500 µg/kg ;
—   de fumonisine B21 (FB2) dans la plage soumise à essai allant de 200 µg/kg à 2 500 µg/kg ;
—   de la toxine T-2 dans la plage soumise à essai allant de 7,5 µg/kg à 360 µg/kg ;
—   de la toxine HT-2 dans la plage soumise à essai allant de 14 µg/kg à 1 800 µg/kg ;
—   de la zéaralénone (ZEN) dans la plage soumise à essai allant de 30 µg/kg à 600 µg/kg ; et
—   de l’ochratoxine A (OTA) dans la plage soumise à essai allant de 10 µg/kg à 230 µg/kg.
dans les céréales et les aliments composés pour animaux à base de céréales, par chromatographie en phase liquide couplée à de la spectrométrie de masse (CL-SM/SM). Il se peut que les plages de travail en routine s’étendent au-delà des plages soumises à essai.

Krma: metode vzorčenja in analize - Določevanje deoksinivalenola, aflatoksina B1, fumonizina B1 in B2, toksinov T-2 in HT-2, zearalenona in ohratoksina A v sestavinah krme in krmni mešanici z LC-MS/MS

Ta analitska metoda evropskega standarda se uporablja za določanje deoksinivalenola (DON) v preskusnem obsegu od 96,2 µg/kg do 3269 µg/kg, aflatoksina B1 (AfB1) v preskusnem obsegu od 2,62 µg/kg do 444 µg/kg, fumonisina B1 (FB1) v preskusnem obsegu od 693 µg/kg do 7529 µg/kg, fumonisina B2 (FB2) v preskusnem obsegu od 203 µg/kg do 2465 µg/kg, T- 2 toksina v preskusnem obsegu od 7,47 µg/kg do 360 µg/kg in HT-2 toksina v preskusnem obsegu od 13,9 µg/kg do 1758 µg/kg, zeralenona (ZON) v preskusnem obsegu od 34,3 µg/kg do 593 µg/kg ter ohratoksina A (OTA) v preskusnem obsegu od 10,8 µg/kg do 228 µg/kg pri žitih in krmnih mešanicah na osnovi žit s tandemsko masno spektrometrijo s tekočinsko kromatografijo (LC-MS/MS). Dejanski delovni obsegi lahko presegajo presušene obsege.

General Information

Status
Published
Publication Date
05-Nov-2019
Withdrawal Date
30-May-2020
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
06-Nov-2019
Due Date
20-Mar-2018
Completion Date
06-Nov-2019

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Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN 17194:2020
01-januar-2020
Krma: metode vzorčenja in analize - Določevanje deoksinivalenola, aflatoksina B1,
fumonizina B1 in B2, toksinov T-2 in HT-2, zearalenona in ohratoksina A v
sestavinah krme in krmni mešanici z LC-MS/MS
Animal feeding stuffs: Methods of sampling and analysis - Determination of
Deoxynivalenol, Aflatoxin B1, Fumonisin B1 & B2, T-2 & HT-2 toxins, Zearalenone and
Ochratoxin A in feed materials and compound feed by LC-MS/MS
Futtermittel: Probenahme- und Untersuchungsverfahren - Bestimmung von
Deoxynivalenol, Aflatoxin B1, Fumonisin B1 und B2, T-2- und HT-2-Toxine, Zearalenon
und Ochratoxin A in Einzelfuttermitteln und Mischfuttermitteln mittels LC-MS/MS
Aliments des animaux : Méthodes d’échantillonnage et d’analyse — Détermination du
déoxynivalénol, de l’aflatoxine B1, de la fumonisine B1 et B2, des toxines T-2 et HT-2, de
la zéaralénone et de l’ochratoxine A dans les matières premières pour aliments et les
aliments composés pour animaux par CL-SM/SM
Ta slovenski standard je istoveten z: EN 17194:2019
ICS:
65.120 Krmila Animal feeding stuffs
SIST EN 17194:2020 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
SIST EN 17194:2020

---------------------- Page: 2 ----------------------
SIST EN 17194:2020


EN 17194
EUROPEAN STANDARD

NORME EUROPÉENNE

November 2019
EUROPÄISCHE NORM
ICS 65.120
English Version

Animal feeding stuffs: Methods of sampling and analysis -
Determination of Deoxynivalenol, Aflatoxin B1, Fumonisin
B1 & B2, T-2 & HT-2 toxins, Zearalenone and Ochratoxin A
in feed materials and compound feed by LC-MS/MS
Aliments des animaux : Méthodes d'échantillonnage et Futtermittel: Probenahme- und
d'analyse - Détermination du déoxynivalénol, de Untersuchungsverfahren - Bestimmung von
l'aflatoxine B1, de la fumonisine B1 et B2, des toxines Deoxynivalenol, Aflatoxin B1, Fumonisin B1 und B2, T-
T-2 et HT-2, de la zéaralénone et de l'ochratoxine A 2- und HT-2-Toxine, Zearalenon und Ochratoxin A in
dans les matières premières pour aliments et les Einzelfuttermitteln und Mischfuttermitteln mittels LC-
aliments composés pour animaux par CL-SM/SM MS/MS
This European Standard was approved by CEN on 9 September 2019.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2019 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 17194:2019 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
SIST EN 17194:2020
EN 17194:2019 (E)
Contents Page
European foreword . 3
1 Scope . 4
2 Normative references . 4
3 Terms and definitions . 4
4 Principle . 4
5 Reagents . 5
6 Apparatus .
...

SLOVENSKI STANDARD
oSIST prEN 17194:2018
01-januar-2018
.UPDPHWRGHY]RUþHQMDLQDQDOL]H'RORþHYDQMHGHRNVLQLYDOHQRODDIODWRNVLQD%
IXPRQL]LQD% %WRNVLQRY7 +7]HDUDOHQRQDLQRKUDWRNVLQD$Y
VHVWDYLQDKNUPHLQNUPQLPHãDQLFL]/&0606
Animal feeding stuffs: Methods of sampling and analysis - Determination of
Deoxynivalenol, Aflatoxin B1, Fumonisin B1&B2, T-2 & HT-2 toxins, Zearalenone and
Ochratoxin A in feed materials and compound feed by LC-MS/MS
Futtermittel - Probenahme- und Untersuchungsverfahren - Multimethode für Mycotoxine
in Einzelfuttermitteln und Mischfuttermitteln mittels LC-MS/MS
Aliments des animaux : Méthodes d’échantillonnage et d’analyse - Détermination du
déoxynivalénol, de l’aflatoxine B1, de la fumonisine B1 et B2, des toxines T-2 et HT-2, de
la zéaralénone et de l’ochratoxine A dans les matières premières pour aliments et les
aliments composés pour animaux par CL-SM/SM
Ta slovenski standard je istoveten z: prEN 17194
ICS:
65.120 Krmila Animal feeding stuffs
oSIST prEN 17194:2018 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
oSIST prEN 17194:2018

---------------------- Page: 2 ----------------------
oSIST prEN 17194:2018


DRAFT
EUROPEAN STANDARD
prEN 17194
NORME EUROPÉENNE

EUROPÄISCHE NORM

November 2017
ICS 65.120
English Version

Animal feeding stuffs: Methods of sampling and analysis -
Determination of Deoxynivalenol, Aflatoxin B1, Fumonisin
B1&B2, T-2 & HT-2 toxins, Zearalenone and Ochratoxin A
in feed materials and compound feed by LC-MS/MS
Aliments des animaux : Méthodes d'échantillonnage et Futtermittel - Probenahme- und
d'analyse - Multiméthode pour la détermination par Untersuchungsverfahren - Multimethode für
CL-SM/SM, des mycotoxines présentes dans les Mycotoxine in Einzelfuttermitteln und
matières premières pour aliments et les aliments Mischfuttermitteln mittels LC-MS/MS; Deutsche und
composés pour animaux Englische Fassung prEN 00327097:2017
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 327.

If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.

Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.


EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2017 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 17194:2017 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
oSIST prEN 17194:2018
prEN 17194:2017 (E)
Contents Page
European foreword . 3
1 Scope . 4
2 Normative reference . 4
3 Terms and definitions . 4
4 Principle . 4
5 Reagents .
...

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